Part:BBa_K3003012:Design

PelB - PenetraMax - Wild Type Insulin with a Linker Peptide ( SCI-57 linker) - Ag43

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 628
  Illegal PstI site found at 1186
  Illegal PstI site found at 1786
  Illegal PstI site found at 2410
  Illegal PstI site found at 2681
  Illegal PstI site found at 2734
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 628
  Illegal PstI site found at 1186
  Illegal PstI site found at 1786
  Illegal PstI site found at 2410
  Illegal PstI site found at 2681
  Illegal PstI site found at 2734
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 100
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 628
  Illegal PstI site found at 1186
  Illegal PstI site found at 1786
  Illegal PstI site found at 2410
  Illegal PstI site found at 2681
  Illegal PstI site found at 2734
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 628
  Illegal PstI site found at 1186
  Illegal PstI site found at 1786
  Illegal PstI site found at 2410
  Illegal PstI site found at 2681
  Illegal PstI site found at 2734
  Illegal NgoMIV site found at 54
  Illegal NgoMIV site found at 454
  Illegal NgoMIV site found at 1678
  Illegal NgoMIV site found at 2188
  Illegal NgoMIV site found at 2209
  Illegal AgeI site found at 1204
  Illegal AgeI site found at 1948
  Illegal AgeI site found at 2362
  Illegal AgeI site found at 2604
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

None.

Source

Cloned from its components.

References

Hua, Q. X., Nakagawa, S. H., Jia, W., Huang, K., Phillips, N. B., Hu, S. Q., & Weiss, M. A. (2008). Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapeutic implications. The Journal of biological chemistry, 283(21), 14703–14716. doi:10.1074/jbc.M800313200